[关键词]
[摘要]
通过对武威市凉州区人参果感病植株所表现出来的花叶、皱缩、植株矮小等症状观察,初步判断可能感染了马铃薯M病毒(PVM)。结合分子生物学手段,提取感病人参果叶片总RNA,采用RT-PCR方法扩增到一段长300 bp的基因序列,连接至PMD19-T载体并测序。测序结果同GeneBank中PVM CP基因序列[GI:361071296]同源性达到了98.97%,说明成功克隆到了PVM基因片段, 即供试人参果确实感染了PVM病毒。
[Key word]
[Abstract]
The mosaic, wrinkled, small plant and other symptoms of susceptible plants of Liangzhou ginseng fruit were observed, the auditor should make a pre-judgment that may be infected with potato virus M(PVM). Experiments combined with molecular biology methods and extract leaves total RNA of the patient feeling of ginseng fruit, it was amplified by RT-PCR method to a long 300 bp gene sequences and connected to PMD19-T vector and sequenced. The Sequencing results showed that homology reached 98.97%with the GeneBank PVM CP gene sequence [GI: 361071296], it can explain gene fragment was cloned into the PVM successful, that the test does ginseng fruit infected with PVM virus.
[中图分类号]
S432.41
[基金项目]
甘肃省科技支撑计划“用融合基因dsRNA获得抗病性人参果种质资源的研究” (1011NKCA077)部分内容;甘肃省农牧厅农业生物技术专项“导入甜蛋白基因创造甜味人参果种质研究”(GNSW-2012-22)部分内容