以陇薯10号试管苗茎段为外植体，分别在5种培养基上进行愈伤组织诱导，比较接种后28 d时的出愈率及愈伤组织的形态，并将胚性愈伤接到10种不同分化培养基上进行再生苗分化。结果表明:接种28 d后，5种培养基均可诱导出大量的愈伤组织，MS+2.0 mg/L 6-BA + 0.2 mg/L NAA + 3.0 mg/L GA3 +0.5 mg/L 2，4-D培养基上胚性愈伤诱导率最高，生长状态最好。胚性愈伤在MS+2.0 mg/L 6-BA+2.0 mg/L ZT + 3.0 mg/L GA3培养基上分化率最高，且分化苗粗壮。

To establish high efficient regeneration system of new potato (Solarium tuberosum L .) variety Longshu 10， stem segments of Longshu 10 tube seedling were inoculated on 5 kinds of culture media to induce callus. After 28 d from inoculation， the frequency of callus induction and callus morphology were compared. The embryonic callus was inoculated on 10 different differentiation media for differentiation of seedling . The results showed that after 28 d from inoculation， a lot of callus were induced from all 5 kinds of media . The frequency of embryonic callus from the medium MS+2.0 mg/L 6-BA + 0.2 mg/L NAA + 3.0 mg/L GA3 +0.5 mg/L 2，4-D was highest， and the growth conditions of embryonic callus was best . The frequency of differentiated seedlings from the medium MS+2.0 mg/L 6-BA + 2.0 mg/L ZT + 3.0 mg/L GA3 was highest， and the differentiation stout seedlings was thick.

S544.1

国家自然科学基金项目“转录因子DREBIA基因的克隆及马铃薯抗旱种质的创制”(31060200)部分研究内容