以分离自葡萄叶片内生细菌菌株Zyx-3为研究对象，依据其形态特征、生理生化特性等初步确定了其分类地位，并采用平板对峙法测定了其对14种植物病原真菌的抑菌能力。结果表明，Zyx-3菌体杆状，中生芽孢，革兰氏染色阳性；菌株具有运动性和需氧性，可以使明胶液化、淀粉水解，接触酶反应阳性，具有耐盐性，可在10% NaCl培养液中生长。根据其形态特征及生理生化特性，初步确定其为芽孢杆菌(Bacillus spp.)。该菌株对玉米大斑病菌(Exserohilum turcicum)、辣椒炭疽病菌(Colletotrichum capsici)、苹果腐烂病菌(Valsa mali)、马铃薯立枯丝核病菌(Rhizoefonia solani)、半夏早疫病菌(Alternaria sonali)、苹果轮纹病菌(Physalospora piricola)、葡萄灰霉病菌(Botrytis cinerea)和葡萄白腐病菌(Coniothyrium diplodiella)、茄子菌核病菌(Sclerotinia sclerotiorum)等14种供试植物病原真菌均有不同程度的抑制作用，抑菌谱广。

The strain Zyx-3 was isolated from grape leaves endophytic bacteria as research object， on the basis of their morphological characteristics， physiological and biochemical characteristics were confirmed its classification status， and the 14 kinds of plant pathogenic fungi inhibitory ability were determined by plate confrontation method. The results shows that the strain Zyx-3 are also rod -shaped， grown in the middle at the end the character of movement quality and aerobic， can make the gelatin liquefaction， starch hydrolysis， contact enzyme reaction is positive， with salt tolerance， can be cultured in the culture medium 10% NaCl. According to its morphological， physiological and biochemical characteristics of cell， initially identified as Bacillus (Bacillus spp.). For 14 kinds of plant pathogenic fungi tested， their have inhibition of different degree， Broad spectrum antimicrobial which the strain of Exserohilum turcimali， Colletotrichum capsici， Valsa mali， Rhizoefonia solani； Alternaria sonali； Physalospora piricola， Botrytis cinerea and Coniothyrium diplodiella， Sclerotinia sclerotiorum .

S182；S663.1

公益性行业(农业)科研专项资助项目(201203035)、甘肃省科技支撑计划项目(1204NKCA099)、甘肃省农业科学院青年基金项目(2013GAAS35)部分内容