采用YEP1和YEP2培养基培养番茄黄化曲叶病毒侵染性克隆pBinPLUS-1.7A+2β，并用固体菌落、液体菌菌液浓度OD600≈0.6、液体菌菌液浓度OD600≈1.0对感病番茄品种MM、中蔬4号、98-B1在4～6叶苗期进行接种处理。结果显示，2种液体培养基的液体菌菌液浓度为OD600≈0.6时接种处理发病率较高，并初步确立了无TY发病地区侵染性克隆接种鉴定方法。

The pBinPLUS-1.7A+2β was nourished in two species medium， and set up three different treatments respectively， such as colonies， the two concentration of agrobacterium is about OD600≈0.6 or OD600≈1.0 . The 4~6 leaves seedlings were inoculated in susceptible tomato varieties MM， Zhongshu 4， and 98-B1 . The results shows that it was higher TY incidence when the two concentration of agrobacterium is about OD600≈0.6 . The techniques of pBinPLUS-1.7A+2β inoculation identification is established preliminarily in the areas， where the TY has not happened .

S436.412.1

甘肃省农业科学院农业科技创新专项(2012GAAS15-16)部分研究内容