采用苦参种子为外植体，以MS为基本培养基，添加不同浓度的NAA和6-BA，进行苦参组培快繁技术研究。结果表明，采用75%乙醇8 s＋0.1%升汞8 min对苦参种子消毒后，污染率低，易获得无菌材料。MS培养基中分别添加0.1 mg/L 、3.0 mg/L 6-BA 和0.5 mg/L NAA、3.0 mg/L 6-BA的组培苗增殖效果较好，增殖率最高可达120.2%；在1/2 MS培养基中添加0.1 mg/L的NAA，更利于苦参组培苗生根。根长1～2 cm的无菌苗，在自然光下驯化培养5 d后移栽至蛭石基质中成活率较高。

In this experiment， seeds have been used as explants， MS as the basic medium adding NAA and 6-BA on different concentrations to research rapid propagation technology of Sophora flavescens var . The results shows that the best method of sterilization for seeds is 70% ethanol (8 s)+0.1% HgCl2 (8 min) which have the lowest contamination rate and the highest seedling rate . The optimal culture medium for subculture is MS+0.1 mg/L NAA+3.0 mg/L 6-BA and MS+0.5 mg/L NAA+3.0 mg/L 6-BA， the highest multiplication rate is 120.2%， 1/2 MS+0.1 mg/L NAA is the best medium for rooting culture . The tissue culture seedling have 1～2 cm roots could be domesticated with natural light for 5 days， and then transplanted in vermiculite have higher survival rate .

S567.5

甘肃省科技攻关资助项目“苦参质量标准和规范化栽培(GAP)研究”(2GS064-A43-019-08)；甘肃省教育厅导师基金项目“苦参组织培养及快速繁殖技术体系研究”(1119-02)部分内容