以当年产当归种子为材料，分别对种子进行去翅和不去翅处理，观察其无菌苗培养条件及愈伤组织诱导、继代培养情况。结果表明，当归种子去翅后，在1/2 MS培养基上有利于获得无菌苗，发芽率达55.4%，污染率仅为6.5%。叶柄为当归愈伤组织诱导的最佳外植体，H培养基为当归诱导愈伤组织的适宜基本培养基。诱导愈伤组织培养基为H+0.5 mg/L 2，4-D时，出愈率达90%以上；继代增殖培养基为H+0.5 mg/L 2，4-D+1.0 mg/L IAA时，增殖效果较好。

The seeds peeled and non-peeled of Angelica sinensis are used to study the influence of different hormone combinations and media on callus induction and plant regeneration. The result shows that seeds peeled on 1/2 MS culture medium to cultivate， germination rate is 55.4%， and the pollution rate is only 6.5%； Petiole are the optimal explant， and H medium are the optimal medium； the optimal medium for callus induction is H+0.5mg/L 2，4-D， and the callus forming rate could reach to 90%； the optimal medium for induction of subculture is H+0.5 mg/L 2，4-D+1.0 mg/L IAA， and the effect of proliferation is better .

S567.23

甘肃省农业科技创新专项(2011GAAS06-8、2012GAAS05-1、2013GAAS03-2、2013GAAS03-2)；甘肃省农业生物技术研究与应用开发项目(GNSW-2011-06)