以马铃薯品种LK99试管苗茎段为外植体，分别在6种培养基上进行愈伤组织的诱导，比较接种28 d时的出愈率及愈伤组织的形态，并将胚性愈伤接到8种不同分化培养基上进行再生苗的分化。结果表明，在接种28 d后，6种培养基均可诱导出大量的愈伤组织，以MS＋2.5 mg/L 6-BA＋0.5 mg/L NAA＋3 mg/L GA3＋0.5 mg/L 2，4-D培养基上胚性愈伤诱导率最高，生长状态最好。胚性愈伤在MS＋2.5 mg/L 6-BA＋1.0 mg/L ZT＋3.5 mg/L GA3培养基上分化率最高，并且分化苗键壮。

To establish high efficient regeneration system of potato (Solarium tuberosum L .) variety LK99， stem segments of LK99 tube seedlings are inoculated on 6 kinds of culture media to induce callus . After 4 weeks from inoculation， the frequency of callus induction and callus morphology are compared . The embryonic callus is inoculated on 8 different differentiation media for differentiation of seedlings . The result shows that after 4 weeks from inoculation， a lot of callus are induced from all 6 kinds of media . The frequency of embryonic callus from the medium E(MS+2.5 mg/L 6-BA+0.5 mg/L NAA+3 mg/L GA3+0.5 mg/L 2，4-D)is highest， and the growth conditions of embryonic callus is best . The frequency of differentiated seedlings from the medium(MS+2.5 mg/L 6-BA+1 mg/L ZT+3.5 mg/L GA3)is highest， and the differentiation seedlings is stout .

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国家自然科学基金(31060200)；甘肃省自然科学基金项目(145RJZA088)；甘肃省农业科学院中青年基金(2014GAAS20)部分内容