以黄色短杆菌FY-18为出发菌株，利用摇瓶发酵生产L-亮氨酸，并利用正交试验和单因素试验分别对其发酵培养基和基本发酵条件进行研究，从而优选出最佳培养基组分和培养条件参数。结果表明，L-亮氨酸发酵培养基的最适配比为:葡萄糖160 g/L、豆粕水解液20 g/L、玉米浆20 g/L、毛发粉15 g/L、硫酸铵70 g/L；其发酵最佳培养条件为:初始pH 7.2，培养温度32 ℃，发酵接种量为10%。在上述最佳条件下摇瓶中发酵的产酸量为22.5 g/L。

Brevibacterium flavum FY-18 was used as original strain to produce L-leucine by shaking flask fermentation. The orthogonal experiments and single factor experiments were adopted to optimize the fermentation culture medium and conditions of L-leucine. The results indicated that the optimized compositions of medium were 160 g/L glucose， 20 g/L soybean meal hydrolysate， 20 g/L corn steep liquor， 15 g/L hair powder and 70 g/L ammonium sulfate， and the fermentation conditions were initial pH at 7.2， 32 ℃ and inoculum 10%. Under the optimized fermentation conditions the production of L-leucine was up to 22.5 g/L .

TQ922