以来自鹿粪便中1株芽孢杆菌为试验菌株，在分子水平上通过16S rDNA同源性序列分析、比较、鉴定，并通过形态观察、革兰氏染色、芽孢染色、生理生化实验，初步确定该菌株为枯草芽孢杆菌J-2。分析影响芽孢杆菌J-2产生抗真菌肽的主要因素，通过正交优化实验得出，芽孢杆菌J-2产生抗真菌肽的最佳发酵条件为500 mL的锥形瓶中加入150 mL初始pH为7.0的PDL(200)培养基，接入9%的液体菌种，在温度为37 ℃条件下发酵24 h。

In this study， with a strain of bacillus from deer feces as the test strains， we identified it as Bacillus subtilis J-2 by morphology， gram stain， spore stain， physiological and biochemical experiments， and it is analyzed， compared and identified by 16S rDNA sequence at the molecular level. the major factors which impact Bacillus J-2 generating antifungal peptides is analyzed by orthogonal optimization experiments， the optimum fermentation conditions of Bacillus J-2 generating antifungal peptides is derived， in 500 mL erlenmeyer flask is added 150 mL initial pH 7.0 PDL(200) medium access 9% liquid spawn， at the temperature of 37 ℃ for fermentation. 24 h.

S852.61

国家自然科学基金“Rib蛋白在罗伊氏乳杆菌肠道粘附中的功能研究”(31360022)