[关键词]
[摘要]
以马铃薯叶片为试验材料，采用改良CTAB法和试剂盒法提取马铃薯叶片基因组DNA。结果表明，2种方法均可得到满足SSR-PCR扩增要求的DNA，其中试剂盒较改良CTAB法提取的DNA质量高、杂质少，DNA得率低，操作简单，耗时短，毒害小，但费用较改良CTAB法高。实践中可根据条件选择适宜方法提取马铃薯基因组DNA。
[Key word]
[Abstract]
The potato leaves as test materials， improved CTAB method and plant genomic DNA extraction Kit is used to extract genomic DNA from leaves of potato in field respectively. The result shows that DNA extacted by the two methods also met demands for SSR-PCR amplification； The quality of DNA obtained by plant genomic DNA Kit is better but the achievement rate of DNA is lower. Moreover， the method of plant genomic DNA Kit is much easier， needed shorter time， had lower toxicity， and high cost than improved CATB method. Suitable method can be choose to genomic DNA of potato according to the actual conditions of the laboratory.
[中图分类号]
S532
[基金项目]
国家自然科学基金“四倍体马铃薯分子遗传图谱的构建与淀粉含量等重要农艺性状的QTL定位研究” (31160299)；甘肃省省青年科技基金计划“甘肃省马铃薯主栽品种指纹图谱的构建及遗传多样性分析”(1308RJYA047)；甘肃省农业科学院农业科技创新专项“甘肃省马铃薯主栽品种及重要种质资源DNA指纹图谱的构建”(2013GAAS43)部分内容
