采用浸虫法测定了10%吡虫啉可湿性粉剂、40%辛硫磷乳油及48%毒死蜱乳油对异迟眼蕈蚊3龄幼虫的毒力，并计算致死中浓度LC50、亚致死剂量LC10、LC20和LC30。结果表明，3种药剂LC10、LC20及LC30对异迟眼蕈蚊的化蛹率、羽化率和单雌平均产卵量具有显著抑制作用，且随着亚致死浓度的增大而增强。10%吡虫啉可湿性粉剂的3种亚致死剂量对异迟眼蕈蚊的抑制作用最小，48%毒死蜱乳油的3种亚致死剂量对异迟眼蕈蚊的影响最大。在3种药剂的亚致死剂量中，对单雌平均产卵量影响最大的为40%辛硫磷乳油，其次是48%毒死蜱乳油和10%吡虫啉可湿性粉剂。除蛹重与对照相比变化幅度不大，其余生物学指标均总体低于对照。

The method of immersing the insects in insecticides used to do the toxicity determination and sublethal testing of 3rd instar larvae of Bradysia difformis. Sublethal dose LC10、 LC20、 LC30 had been treated the insects， and then after 24 h survival larvae would breeded in regulated heating block(temperature 25 ℃， humidity 70%~80%). Biological parameters are recorded， such as pupation rate， pupa weight， emergence rate and eggs laid per female. The result indicates that three kinds of insecticides all had inhibiting effect， for 48% Chlorpyrifos EC the sublethal effects is the best， and 10 % Imidacloprid wettable powder is the worst one. In the sub lethal dose of three kinds of insecticides， 40% Phoxim EC the average single female eggs effect is the best， followed by 48% Chlorpyrifos EC and 10% Imidacloprid wettable powder， Compared with control， the pupa weight didn't change obviously， but other biological parameters declined especially the eggs laid per female.

S436.33

公益性行业(农业)科研专项(201303027)