采用RAPD分子标记技术，对甘肃省定西市农业科学研究院选育的板蓝根新品系BLG2012-04和当地大田栽培种进行 PCR 扩增反应，以鉴别其遗传多样性和亲缘关系。结果显示，对于BLG2012-04和当地大田栽培种，筛选出的11条引物均扩增出119个位点，平均每条引物能扩增出10.8个位点。两者之间的主谱带基本一致，说明它们的遗传背景具有很大的相似性；次谱带存在不同程度的差异，这在分子水平上说明BLG2012-04与当地大田栽培种具有一定的遗传差异性。

In this study， RAPD molecular marker technology are used for PCR amplification reaction of the two Radix Isatidis varieties BLG2012-04 and main cultivar， to identify two Radix Isatidis varieties of lines between genetic diversity and genetic relationship. The result shows that a total of 119 DNA bands are amplified by 11 oligonucleotide primers. The average each primer can amplification out 10.8 sites. The Lord band of two Radix Isatidis are basically identical that the genetic background of between new lines and main cultivar had great similarity， but subband have difference of different degree， the two lines has certain genetic differences.

S567.23

2013年中医药部门公共卫生专项—“国家基本药物所需中药材种子种苗繁育基地建设”(国中医药办规财发[2013]41)部分研究内容