以吉木萨尔白蒜为试验材料，利用大蒜鳞茎盘为外植体，在B5+6-BA0.5 mg/L+NAA0.5 mg/L+蔗糖30 g/L+琼脂4.5 g/L培养基进行初始培养获得绿芽，在此基础上开展试管内诱导微鳞茎培养基配方研究。结果表明，1/2 MS＋IBA 1.5 mg/L＋NAA 0.1 gm/L＋蔗糖50 g/L＋琼脂4.5 g/L为组培苗试管内诱导鳞茎较好的培养基配方，在该培养基上大蒜绿芽第45 天开始形成鳞茎，第90 天鳞茎直径为0.3～0.5 cm。

The Jimsar white garlic as experimental materials， using garlic bulb as explants， Initial culture is carried out to obtain green bud in B5+6-BA 0.5 mg/ L +NAA0.5 mg/L + sucrose 30 g/L + agar 4.5 g/L medium， on the basis of this study， the formula of the culture medium for inducing the micro bulb in vitro is developed. The result shows that 1/2 MS＋IBA 1.5 mg/L＋NAA 0.1 gm/L 1/2 MS + IBA 1.5 mg/L + sugar + 50 g/L + agar 4.5 g/L is a better culture medium formula for inducing bulb in tissue culture plantlet， the garlic green shoots began forming a bulb the in 45th day， the bulb diameter up to 0.3～0.5 cm in 90th day under the culture medium.

S633.4

甘肃省科技支撑计划项目“陇东南大蒜脱毒快繁技术研究及示范”( 1204NKCE105)部分内容