采用感官评吸的方法，对从陈化烟叶上初筛分离获得的224株产淀粉酶菌株进行复筛，得到3株能显著改善烟叶品质的产淀粉酶菌株A-7、A-8和A-9。对不同浓度酶液改善烟叶品质的分析表明，0.29 U/mL的酶液A-7、80.67 U/mL的A-8、213.33 U/mL的A-9对烟叶评吸指标有所改善。以菌株A-8为进一步研究对象，对其产酶条件进行了优化。结果表明，A-8在37 ℃、pH 6.0的条件下，震荡培养36 h生物量达到最大，60 h酶活达到最高，发酵条件的优化可使其单位酶活力提高2.7倍。

Based on 224 of amylase-producing bacterial strains isolated from tobacco leaves and their screening for the production of amylase in our previous work， this study aimed to screen amylase-producing bacterial strains which could obviously improve tobacco interior quality by the assessment of sense organ wih smoking， including amylase A-7， A-8， A-9. Different concentrations of the enzyme solution to improve the quality of tobacco leaves shows that 0.29 U/mL of the enzyme solution A-7， 80.67 U/mL of A-8， 213.33 U/mL of A-9 on tobacco smoking indicators have improved. The conditon for enzyme production from strain amylase-producing bacterial A-8 is optimized. The result shows that A-8 at pH 6.0， 37 ℃ conditions， shaking culture 36 hours biomass maximum， 60 hours highest activity， the total enzyme activity could be improved by 2.7 times under the optimum conditions.

S572

云南中烟工业有限责任公司项目(S-6013067)部分内容。