以兰州百合鳞片和叶片为试材，采用改良CTAB法获得了高质量的基因组DNA。为优化兰州百合RAPD-PCR反应体系，利用正交试验设计，对兰州百合RAPD-PCR反应体系中的5种主要因素进行4水平共计16个组合设计，并选用3种反应程序，通过琼脂糖凝胶电泳结果分析，最终获得最佳反应体系为:20 uL反应体系中含30 ng模板DNA、0.5 μmol/L随机引物、0.2 mmol/L dNTPs、2.25 mmol/L MgCl2， 及1.5U Taq DNA聚合酶。最佳反应程序:94 ℃预变性4 min；94 ℃变性1 min，36 ℃退火1.5 min；72 ℃延伸2 min；35个循环；最后72 ℃延伸7 min。

High quality genomic DNA is obtained from lily bulbs and leaves using improved CTAB method. In order to get reliable and repeatable reaction system of RAPD-PCR amplification for Lanzhou lily， we optimized the RAPD-PCR reaction system at 4 levels and 5 factors by orthogonal experimental design. The optimized RAPD-PCR system is tested on 3 PCR amplification procedure. The result of polyacrylamide gel electrophpresis shows that the best reaction system is 20 ul reaction solution， which contained 30 ng template DNA， 0.5 μmol/L primers， 0.2 mmol/L dNTPs， 2.25 mmol/L MgCl2 and 1.5 U Taq DNA polymerase. The reaction procedure is as follows: pre-denaturing at 94 ℃ for 4 min， 35 cycles of denaturing at 94 ℃ for 1 min，annealing at 36 ℃ for 1.5 min and extending at 72 ℃ for 2 min， extending at 72 ℃ for 7 min after the cycles.

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甘肃省农业科学院青年基金项目(2013GAAS28)；甘肃省农业科学院科技创新工程学科团队项目(2015GAAS02)。