[关键词]
[摘要]
以红树莓品种海尔特兹和秋福的单芽茎段外植体为试验材料,研究了树莓组培快繁各阶段的最佳培养基及出瓶移栽基质。结果表明,初代培养基为MS+6-BA 0.1 mg/L时两个红树莓品种的诱导腋芽萌发效果最好,MS+6-BA 0.5 mg/L+NAA 0.1 mg/L为最佳继代增殖培养基。海尔特兹的最佳生根培养基为1/2MS+IBA 0.1 mg/L+NAA 0.05 mg/L,秋福的最佳生根培养基为1/2MS+IBA 0.2 mg/L+NAA 0.1mg/L。最佳出瓶移栽基质为蛭石、河沙、育苗基质、园土按体积比0.15 ∶ 0.15 ∶ 0.2 ∶ 0.5配制。
[Key word]
[Abstract]
For the purpose of rapid propagation of 2 cultivars of red raspberry Heritage and Autumn Bliss, media of each propagating stage in vitro and transplanting in soil are optimized and compared. Single~bud~stem cuttings of the two cultivars are used as initial explants in this research. The result shows that the highest initial sprouting rates are achieved with the both varieties on a MS medium supplemented with 6-BA at 0.1 mg/L; for shoot multiplication, a medium composition as MS+6-BA 0.5 mg/L+NAA 0.1 mg/L is the optimal. The best rooting medium for Heritage is 1/2MS+IBA 0.1 mg/L+NAA 0.05 mg/L, while the highest rooting ratio for Autumn Bliss was observed on a medium 1/2MS+IBA 0.2 mg/L+NAA 0.1 mg/L. Shoots developed good roots invitro are then transplanted into soil and a mixture of 15% vermiculite+15% sand+20% nursery matrix+50% garden soil is the best transplanting matrix.
[中图分类号]
S723.1
[基金项目]