以采自静宁的葫芦巴为材料，研究了1 mol/L的盐酸在60 ℃下的不同解离时间对根尖染色体制片的影响，并确定染色体数目和进行核型分析。结果表明，静宁葫芦巴根尖最佳解离时间为2.0～2.5 min。染色体数为16，核型公式为2n＝2x=16=8m+8sm(2SAT)，臂比值变化在1.07～2.88，平均臂比为1.926，随体在第6对染色体短臂上，核型类别为2A。

In this paper， the effects on routine chromosome preparation of Trigonella foenum-graecum L. with different acidolysis time with 1 mol/L HCl at 60 ℃ are studied. The chromosome number and the karyotype are investigated with conventional plant root tip squashing method. The result shows that the best acidolysis time is 2.0～2.5 min. The chromosome number is 16， with karyotype formula as follows: (2n)=2x=16=8m+8sm(2SAT). The change of ration of chromosome length(L/S) is between 1.07～2.88， with an average ration is 1.926， and a pair of satellites in the short arm of 6th chromosomes， The karyotype type is 2A.

Q343.2

甘肃省中药材产业科技攻关项目(GYC12-06)。