以青花菜F1代杂交种绿雄60为材料，研究了青花菜小孢子单核靠边期花蕾的特征。结果表明，通过整枝可以得到发育整齐的青花菜小孢子花蕾，当花蕾长度为3.70～4.70 mm时，单核靠边期小孢子比例最高，此时花蕾为绿色，花药黄绿色，花瓣/花药为0.83～1.00。以上指标可以作为青花菜小孢子取样的参考指标。

Lüxiong 60 of Brassica oleracea L as the experimental material， the characteristics of mid-late uninucleate stage of microspore bud side is researched. The result shows that the training can get a neat microspore bud development， when the bud length is 3.70 ~ 4.70 mm， mononuclear side has the highest percentage of microspore stage， the buds of green， anthers yellow-green， petals/anther ratio is 0.83 ~ 1.00. These indicators can be a reference index of broccoli microspore sampling， provide the basis for accurate sampling.

S635.3

甘肃省自然科学基金(1506RJZA196)、甘肃省农业科学院中青年创新基金(2016GAAS47)、农业部园艺作物生物学与种质创制西北地区科学观测试验站项目(2015-A2621-620321-G1203-066)部分内容。