[关键词]
[摘要]
以4个酿酒葡萄品种(北醇、梅鹿辄184、黑比诺222、品丽珠)及4种砧木(贝达、SO4、Ln33、LDP294)为试验材料,考察不同低温处理(0、-7、-15、-20、-30、-70 ℃)对CAT活性、POD活性、PPO活性、SOD活性、MDA含量、可溶性糖含量及Pro含量等生理指标的影响,并通过隶属函数法、生理指标的相关性及主成分分析对酿酒葡萄的抗寒性进行综合性评价。结果表明:经隶属函数统计分析,8个酿酒葡萄及砧木品种的综合抗寒性从高到低依次为贝达、Ln33、LDP294、北醇、SO4、黑比诺222、梅鹿辄184、品丽珠。对7个抗寒性指标的相关性分析显示,在葡萄抗寒性鉴定中,不能单独依赖某个或某两个指标对葡萄抗寒性进行评价;各测定指标主成分分析显示,CAT活性、POD活性、PPO活性及MDA含量具有绝对值较大的特征向量,贡献率达48.66%。
[Key word]
[Abstract]
Effect of different low temperature (0、 -7、 -15、 -20、 -30、 -70 ℃) on physiological indexes (Activity of CAT, POD, PPO, SOD and the content of MDA, sugar, soluble Pro) is studied on the 8 different Cultivas of wine grape(54-4-271, Merlot 184, Pinot Noir 222, Cabernet Franc) and rootstock(Beta, SO4, Ln33, LDP294) , comprehensive evaluation of cold tolerance is treated by the methods of subordinate function, correlation analysis of physiological indexes and principal component analysis. The result shows that the ability of cold resistance from high to low as follows Beta、 LDP294、 Ln33、 SO4、 Pinot Noir 222、54-4-271、 Cabernet Franc、 Merlot 184. The correlation analysis of 7 different physiological indexes indicated that the cold resistance could not be evaluated by relying on one or two physiological indexes. Principal component analysis of 7 different physiological indexes stated that the activities of CAT, POD, PPO and the content of MDA had absolute value of a larger feature vector; the contribution rate reached 48.66%.
[中图分类号]
S663.1;Q142
[基金项目]
国家自然科学基金(31360298);甘肃省干旱生境作物学——省部共建国家重点实验室开放基金(GSCS-2012-07)部分内容。