采用水蒸气蒸馏法从珠光香青中提取挥发油，采用GC-MS方法分析其化学组成，并进行珠光香青挥发油对赤拟谷盗触杀和趋避的生物活性的测试。珠光香青挥发油的主要化学成分有石竹烯氧化物(35.94%)、八氢1，7-二甲基-4-(甲基乙烯基)-1，4-桥亚甲基-1氢-茚(11.84%)、2-异丙基-5-甲基-9-亚甲基-双环[4.4.0]-1-癸烯(11.78%)、桉油烯醇(7.91%)、喇叭茶醇(7.88%)、α-毕橙茄醇(6.25%)。生物活性测试结果显示，珠光香青挥发油对赤拟谷盗触杀的LD50值为41.25 mg/头，同时珠光香青挥发油对赤拟谷盗具有很强的趋避活性。

The essential oil is extracted from Anaphalis margaritacea by steam distillation method， and the components of essential oil is analyzed by GC-MS method. Repellency and contact activity of the essential oil against Tribolium castaneum are tested. The main components of essential oil of A. margaritacea are caryophyllene oxide (35.94%)， Alloisolongifolene (11.84%)， 2-isopropyl- 5-methyl-9-methylene-Bicyclo [4.4.0] dec-1-ene (11.78%) and espatulenol (7.91%)， palustrol (7.88%)， α-cadinol (6.25%). The result of the biological activity test shows that the LD50 value of the contact toxicity of the essential oil against T. Castaneum is 41.24 mg， and the essential oil possessed stronger repellency activity against T. Castaneum.

S379.5