[关键词]
[摘要]
以马铃薯品种陇薯3号及其转AtDREB1A基因株系T1为研究对象,在干旱胁迫条件下,研究了AtDREB1A基因过量表达对盛花期生理生化指标的影响。分析表明,在不同程度的干旱胁迫下,转基因株系T1盛花期叶片的超氧化物歧化酶(SOD)活性及脯氨酸(Pro)含量均高于同期非转基因陇薯3号对照,而丙二醛(MDA)含量和相对电导率均低于陇薯3号。以上结果初步表明,AtDREB1A基因的过量表达明显提高了马铃薯对干旱的抗性。
[Key word]
[Abstract]
In order to study the effects of excessive expression of AtDREB1A gene on physiological and biochemical indexes of potato at flowering stage, the cultivar of potato Longshu 3 and its AtDREB1A genetic strain T1 is used as materials and the activity of SOD, the content of Pro, the content of MDA and the electrical conductivity in leaves are analyzed. The result shows that the activity of SOD and the content of Pro in the transgenic potato were higher than those in the non-transgenic control Longshu 3, and the content of MDA and the electrical conductivity in transgenic potato are lower than those of Longshu 3. The result shows that the excessive expression of AtDREB1A gene in potato improved the potato drought-resistance.
[中图分类号]
S532
[基金项目]
“国家自然科学基金”项目(31560412)、 “国家马铃薯产业技术体系”项目(CAR-10-P05)资 助。