采用离体叶盘法测定了不同来源葡萄霜霉病菌对4种杀菌剂的敏感性。结果表明，采自宁夏金沙林场的霜霉菌EC50由大到小依次为96%霜脲氰、96.4%甲霜灵、97.5%烯酰吗啉、95%醚菌酯；采自天水市麦积区社棠镇霜霉菌的EC50由大到小依次是96%霜脲氰、96.4%甲霜灵、95%醚菌酯、97.5烯酰吗啉；采自甘肃省农业科学院葡萄园的霜霉菌(兰州菌株)EC50由大到小依次是96%霜脲氰、95%醚菌酯、97.5%烯酰吗啉、96.4%甲霜灵。各地菌株对96%霜脲氰均不敏感，对95%醚菌酯和97.5%烯酰吗啉较敏感。兰州菌株对96.4%甲霜灵很敏感，这可能与菌株采集地使用该类药剂较少有关。

The sensitivity to fungicides of Plasmopara viticola isolates collected from different regions is determined using leaf disc method. The result shows that there are some significant differences in sensitivity to dimetho-morph， kresoxim-methyl， cymoxanil， and metalaxyl of P. viticola isolates collected from Ningxia， Tianshui and Lanzhou. The EC50 values of P. viticola isolates collected from Ningxia， from largest to smallest， are cymoxanil， metalaxyl， dimethomorph， and kresoxim-methyl， respectively. The EC50 values of P. viticola isolates collected from Tianshui， in turn， are cymoxanil， metalaxyl， kresoxim-methyl， and dimethomorph， respectively. The EC50 values of P. viticola isolates collected from Lanzhou， from largest to smallest， are cymoxanil， kresoxim-methyl， dimethomorph， and metalaxyl， respectively. On the whole， P. viticola isolates collected from different regions are resistant to cymoxanil and sensitive to kresoxim-methyl and dimethomorph. However， P. viticola isolates collected from Lanzhou are sensitive to metalaxyl， because of using lesser phenyl amide fungicides in collection region.

S436.631

公益性行业(农业)科研专项(201203035)、甘肃省科技支撑计划资助项目(1204NKCA099)、甘肃省农业科学院科技创新专项(2016GAAS08)。