综述了CRISPR/Cas9基因编辑系统及其在水稻育种中的应用。在水稻基因组水平上进行基因定向编辑改造对水稻育种具有重要意义。CRISPR/Cas9基因编辑系统是近几年来研究发现的一种定点基因组编辑新工具，仅需要短RNA和核酸酶就可以对特定的靶标基因进行突变，因其简便高效而被广泛应用于包括水稻在内的多种生物的基因组编辑中。

It is great significance in rice breeding of gene directed to edit on the rice genome level. CRISPR/Cas9 gene editing system is in recent years， the study found that a new fixed-point genome editing tools， need only a short RNA and DNA enzyme can in a specific target gene mutations， because of simple and highly effective and widely used in including rice， a variety of biological genome in the editor. Through the review CRISPR/Cas9 gene editing system and its application in rice breeding， in order to provide some references for the research in this field.

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兰州职业技术学院2016年院内科研项目(项目编号:2016B-2)。