[关键词]
[摘要]
采用盆栽控水试验,研究了对水分敏感性不同的2个玉米品种酒单2号和豫玉22号幼苗在水分胁迫及复水后株高、植株生物量、叶片MDA含量、SOD、POD及CAT等酶活性的变化,以探讨干旱胁迫及复水对玉米生长发育及抗氧化酶的影响机制。结果表明,干旱胁迫可抑制2个玉米品种苗期植株生长,导致株高、地上部生物量显著下降,酒单2号对干旱的敏感性高于豫玉22号。复水后,2个品种的株高、生物量都产生了补偿效应,豫玉22号更明显。干旱胁迫增加了2个品种叶片的丙二醛含量,豫玉22号变化幅度小于酒单2号。复水后豫玉22号叶片中MDA含量更接近于对照水平。干旱胁迫下,豫玉22号通过保持较高的SOD活性来减弱活性氧伤害,而复水后通过较高的SOD、POD协同作用有效清除活性氧,避免对细胞膜造成伤害。
[Key word]
[Abstract]
To discuss the response mechanism of corn growthand antioxidant enzymesto drought and rewatering, using potted water control test. and the plant height, total biomass, the malondialdehyde(MDA) content and antioxidant enzymes including superoxide dismutase(SOD), catalase (CAT), peroxidase (POD) are analyzed in different water sensitivity of Jiudan 2 and Yuyu 22 under drought stress and rewatering. The result shows that drought stress inhibited growth of the two corn cultivars, resulting in the significant decline in plant height and total biomass. The drought sensitivity of jiudan 2 is greater than that of Yuyu 22. After rewatering,the plant height and biomass of the two cultivars produced compensatory effects, and Yuyu 22 is more obvious. MDA in leaf of two cultivars is increased under drought stress, Jiudan 2 shows that larger increase in MDA than Yuyu 22. The content of MDA in Yuyu 22 is more close to the control level after rewatering. Under drought stress,Yuyu 22 can reduce active oxygen damage by keeping the higher SOD activities,and it can remove effectively reactive oxygen species and avoid membrane activities of by the higher SOD, POD and CAT enzymes and their collaboration.
[中图分类号]
S513
[基金项目]
国家自然科学基金(31460323);国家科技支撑计划(2015BAD22B01-05);甘肃省农业科学院农业科技创新专项(2011GAAS06-2-5)。