以余甘子无菌幼苗作为材料，通过正交试验研究影响余甘子愈伤组织诱导的因素，以完善余甘子组织培养体系。结果表明:以茎或叶作为外植体，在MS+1.0 mg/L 2，4-D +0.5 mg/L 6-BA培养基中，愈伤组织诱导率达87.9%以上，愈伤组织生长良好。

Using asepsis seedling of Phyllanthus emblica as material, the factors that affect the callus induced were studied, in order to improve the system of tissue culture for Phyllanthus emblica through orthogonal experiment. The results show that when using stems or leaves as explants, inducement rate of calli was above 87.9% and calli grew well in the medium of MS+1.0 mg/L 2,4-D +0.5 mg/L 6-BA.

Q813.1