利用单倍体诱导系获得玉米单倍体，再加倍获得自交系是当前应用最广泛的玉米育种方法，可以加快玉米育种的进程，提高育种效率，而单倍体加倍方法是其中的关键环节。采用大田注射法研究了秋水仙素不同配方对10个玉米材料的单倍体加倍效果。结果表明，秋水仙素具有很好的加倍作用，剂量以0.06%秋水仙素+2%二甲基亚砜(DMSO)为优，加倍率为6.5%～31.3%，平均加倍率17.47%。

The obtaining of inbred lines from doubled haploid inducers is the most widely used corn breeding method in the world， which can speed up the process of corn breeding and improve the breeding efficiency. The haploid doubling method is one of the key links. Using the injection method in the field， the haploid doubling effects of different colchicine formulations on 10 corn materials were studied. The results showed that colchicine had a good doubling effect with the dosage of 0.06% colchicine + 2% dimethyl sulfoxide (DMSO). The doubling rate ranged 6.5%～31.3%， and the average doubling rate was 17.47%.

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甘肃省科技计划(1504WKCA079)、 (1604JLGA175)。