对从国内征集的8份野生胡麻种质资源进行愈伤组织诱导，筛选最佳诱导培养基。对愈伤组织进行不同浓度NaCl胁迫，测定耐盐型和敏感型野生胡麻愈伤生理指标，评价其耐盐性，以探明其对NaCl胁迫的生理响应。结果表明，对野生胡麻愈伤诱导效果最好的培养基为MS＋NAA(0.15 mg/L)＋6-BA(1.5 mg/L)，愈伤诱导率从大到小依次为乌兰察布蓝花、张家口蓝花、定西蓝花、平凉蓝花、会宁蓝花、兰州蓝花、临洮蓝花、乌兰察布红花。耐盐性由强到弱依次为乌兰察布蓝花、定西蓝花、会宁蓝花、兰州蓝花、张家口蓝花、平凉蓝花、临洮蓝花、乌兰察布红花。耐盐性野生胡麻愈伤组织在NaCl胁迫下SOD、CAT和POD等酶活性较敏感型种质更高，MDA质量分数变化幅度较敏感型种质小，表明耐盐性种质在盐胁迫下膜细胞受盐害程度相对较小，保护酶修复能力强，保护性作用能够持续稳定发挥。

In this study， 8 materials of wild flax were used to induce callus and screen the perfect culture medium； the salt tolerance of these materials were evaluated by NaCl stress conducted on callus； the physiological response of the haloduric and sensitive wild flax were tested， to investigate its physiological response to NaCl stress. The results show that the optimal culture medium to callus induction of wild flax was MS＋NAA(0.15 mg/L)＋6-BA(1.5 mg/L). The callus induction rates ranged from high to low in order of Blue Flower in Wulanchabu， Blue Flower in Zhangjiakou， Blue Flower in Dingxi， Blue Flower in Pingliang， Blue Flower in Huining， Blue Flower in Lintao， and Red Flower in Wulanchabu. The salt tolerance ranged from strong to weak in order of Blue Flower in Wulanchabu， Blue Flower in Huining， Blue Flower in Pingliang， Blue Flower in Lintao， and Red Flower in Wulanchabu. The callus under NaCl stress of haloduric wild flax had higher activities of SOD， CAT and POD， and smaller variation in MDA mass fraction than those of sensitive wild flax， which indicated that the salt-tolerant germplasm had relatively small salt damage to the membrane cells under salt stress， strong protective enzyme repair ability and sustained and stable protective effect.

S563.2

国家自然科学基金(31560347)；国家自然科学基金(31660368)；国家现代农业产业技术体系(CARS-14-1-03)；兰州市人才创新创业项目(2017-RC-97)；甘肃省农业科学院科研条件建设及成果转化项目(2017GAAS22)。