以羧甲基纤维素钠为唯一碳源，从来自甘肃永昌的双孢菇培养料中分离筛选得到1株降解纤维素的耐高温菌X3。利用Biolog GEN III微孔板将该菌株鉴定为芽孢杆菌(Bacillus vallismortis/subtilis)。在初始pH 为7.0、温度为50 ℃、接种量为2%、摇床转速为200 r/min的条件下培养72 h，该菌株粗酶液的CMCase活力达到20.36 U/mL。

Using sodium carboxymethyl cellulose as sole carbon resource， a Hyperthermophilic cellulase strain X3 was isolated from culture medium of Agaricus bisporus in Yongchang Gansu. The strain was identified as Bacillus vallismortis/subtilis with Biolog GEN III micropore plate. The enzyme yield of strain X3 reached 20.36 U/mL after culturing 72 hours under the conditions as follows: initial pH was 7.0，temperature was 50 ℃，inoculum was 2%，rotation speed was 200 r/min.

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兰州市科技支撑计划(2015-3-58)。