以酿酒酵母缺陷型(Saccharomyces cerevisiae)与黏红酵母(Rhodotorula glutinis)为亲本菌株，分别采用聚乙二醇(PEG)和电击方法进行原生质体融合。对得到的融合细胞进行木糖发酵验证，通过检测发酵液中乙醇的含量，筛选出1株产乙醇的重组酵母菌株。利用该重组酵母进行木糖、葡萄糖共发酵，发酵条件为接种量3%，发酵温度27~30 ℃，pH 5.5~6.5，发酵组分为4%木糖、3%蛋白胨、2%酵母浸粉和12%葡萄糖，发酵周期40 h，结果重组酵母可发酵木糖，木糖消耗速率明显低于葡萄糖，乙醇浓度为72 g/L。

In this paper， Saccharomyces cerevisiae and Rhodotorula glutinis were used as parent strains， and protoplast fusion was performed using polyethylene glycol (PEG) and electroporation methods. The resulting fused cells were subjected to xylose fermentation verification. By detecting the ethanol content in the fermentation broth， a high-yield recombinant yeast strain was selected. The recombinant yeast was used for the co-fermentation of xylose and glucose. The fermentation conditions were as follows: inoculation amount 3%， fermentation temperature 27～30 ℃， pH 5.5～6.5， fermentation composition of 4% xylose， 3% peptone， 2% yeast extract and 12% glucose， fermentation cycle 40 h. The experimental results showed that the recombinant yeast could ferment xylose， but the consumption rate of xylose was significantly lower than that of glucose， and the ethanol yield could reach 72 g/L.

TK6；Q939.97；Q815