以甘肃苦水玫瑰外植体为试验材料，研究了苦水玫瑰组培苗的诱导、增殖、继代、生根与移栽技术体系。结果表明，甘肃苦水玫瑰组培苗的诱导与增殖培养基以 WPM+6-BA1.5 mg/L最佳，增殖倍数可达 3.56；继代4次后，丛生芽增殖倍数达到5.43倍。瓶内生根培养基以 WPM+IBA 0.1 mg/L+NAA 0.3 mg/L为佳，生根率可达82.68%。试管苗采用苗床栽培，用腐殖质、田园土和蛭石作基质，在50%遮阴率条件下植株生长量最佳，成活率最高，为91.00%。

With Gansu Kushui rose explants as experimental material, the Kushui rose plantlet induction, proliferation, subculture, rootage and transplant technical system was researched. The results showed that the best culture medium for plantlet induction and proliferation of Gansu Kushui rose was WPM+6-BA1.5 mg/L， the proliferation multiple was 3.56；after subcultured for 4 generations， tufted bud proliferation multiple reached 5.43. The best culture medium of test-tube rooting that were used for rootage was WPM+IBA 0.1 mg/L+NAA 0.3 mg/L, the rootage rate was 82.68%. The highest plant survival rate was 91% when seedlings were cultivated in seedbed， substrate used humus, garden soil and vermiculite under 50% shading.

S685.12

国家重点研发计划项目(2017YFD0201602-4)；甘肃省农业科学院科技支撑计划(2016GAAS03)。