为了解不同红豆草材料在越冬期根系对低温胁迫的响应特征及其抗寒性强弱，以7份红豆草(5份来自俄罗斯、2份来自国内)为供试材料，对红豆草根系可溶性糖含量、可溶性蛋白质含量、脯氨酸(Pro)含量、丙二醛(MDA)含量、过氧化氢酶(CAT)活性、过氧化物酶(POD)活性等6项生理指标的动态变化进行了监测。结果表明，供试红豆草材料根系可溶性糖含量(WWS)、可溶性蛋白质含量(SP)和脯氨酸含量及CAT活性和POD活性均随着气温下降而增加，随气温升高而减少，MDA含量呈下降趋势。经抗寒隶属度分析，得出其抗寒性由大到小的顺序为ZXY2003p-132、ZXY2010p-7369、蒙农红豆草、甘肃红豆草、ZXY2003p-147、ZXY2005p-623、ZXY2007p-3423。从俄罗斯引进的红豆草材料ZXY2003p-132和ZXY2010p-7369比国内红豆草品种蒙农红豆草、甘肃红豆草抗寒性强。

In order to understand the response characteristics and cold resistance of different Onobrychis viciifolia cultivars to low temperature stress during overwintering， with 7 Onobrychis viciifolia cultivars (5 from Russia and 2 from China) being used as material， the dynamic changes of 6 physiological indexes in the roots of Onobrychis viciifolia were monitored， such as the contents of soluble sugar， soluble protein， proline (Pro) and malondialdehyde (MDA)， activity of catalase (CAT) and peroxidase (POD) activity. The results showed that the soluble sugar content (WWS)， soluble protein content (SP)， proline content， CAT activity and POD activity of roots of Onobrychis viciifolia increased with the decrease of air temperature， but decreased with the increase of air temperature， the content of MDA showed a downward trend. According to the analysis of the degree of Cold resistance subordination， the order of cold resistance from large to small was ZXY2003p-132， ZXY2010p-7369， Onobrychis viciaefolia. Scop. cv. Mengnong， Onobrychis viciaefolia. Scop. cv. Gansu， ZXY2003p-147， ZXY2005p-623， ZXY2007p-3423. The materials of Onobrychis viciifolia XY2003p-132 and ZXY2010p-7369 introduced from Russia have stronger cold resistance than domestic cultivars Onobrychis viciaefolia. Scop. cv. Mengnong， Onobrychis viciaefolia. Scop.cv. Gansu.

S541；Q945.7

国家自然基金(31460630)专项资助。