以生防放线菌长枝木霉T6和放线菌ZZ-9为试材，采用圆盘滤膜法和对口培养法比较T6、ZZ-9所产生的代谢物对苹果树腐烂病病菌、苹果树轮纹病病菌和苹果树早期落叶病病菌的拮抗作用，结果表明:长枝木霉T6的非挥发性物质对这3种病原菌抑制效果明显，抑制率分别达到97.50%、83.69%、68.35%。放线菌ZZ-9的非挥发性物质对苹果树腐烂病菌和苹果树轮纹病菌的抑制率达到了96.25%和72.42%%，对早期落叶病菌的拮抗作用较弱，抑制率小于50%。长枝木霉T6和放线菌ZZ-9的挥发性物质对这3种病原菌的拮抗作用不明显，抑制率均小于50%。长枝木霉T6和放线菌ZZ-9对苹果树三种病害的拮抗作用主要由二者所产生的非挥发性物质起作用。

With Trichoderma longibrachiatum T6 and actinomycete ZZ-9 as test materials， the disc filter method and the counterculture method were used to compare antagonistic effects of the metabolites produced by the two biocontrol bacteria to the pathogens of valsa mali， apple white rot and Alternaria mali to provide a basis for spatial distribution of pesticides when these two biocontrol bacteria are used as biocontrol agents to control apple tree diseases. The results showed that the non-volatile substances of Trichoderma longibrachiatum T6 inhibited the three pathogens significantly， and the inhibition rates reached 97.50%， 83.69% and 68.35%， respectively. The inhibitory rate of non-volatile substances of actinomycete ZZ-9 against valsa mali and apple white rot reached 96.25% and 72.42%%， and the antagonism of Alternaria mali was weak， and the inhibition rate was less than 50%; The antagonism of the volatile substances of Trichoderma longibrachiatum T6 and actinomycete ZZ-9 on these three pathogens was not obvious, and the inhibition rate was less than 50%. It is concluded that the antagonistic effects of Trichoderma longiflorum T6 and actinomycete ZZ-9 on three diseases of apple trees mainly play a role in the non-volatile substances produced by the two fungi，which provides important theoretical basis for the development of related preparations in the later stage.

S436.611.1

甘肃省大学生创新创业训练计划项目；甘肃农业大学学生科研训练计划项目(20171106)；甘肃省高等学校科研项目(2017A-028)。