为明确黄芪根腐病致病机制，探索感病植株在物质代谢上对毒素的响应，将分离所得茄病镰刀菌毒素粗提物稀释成不同浓度胁迫处理黄芪幼根，对处理后的幼根蛋白质含量进行了测定。结果表明，随着毒素胁迫时间的延长，黄芪幼根蛋白质含量均快速下降，这可能是随着毒素作用时间的延长，破坏了植株体内的正常代谢，进而抑制了蛋白质的合成。毒素胁迫后黄芪幼根可溶性糖含量降低得快，说明植株体内呼吸作用加强；脯氨酸含量则随着胁迫时间和毒素浓度的增加而增加，说明黄芪幼苗对毒素危害表现为敏感响应。

In order to clarify the pathogenesis of astragalus root rot disease and explore the response of susceptible plants to toxins in terms of material metabolism， the crude extract of Fusarium solani toxin from the eggplant was diluted into different concentration for stress treatment to astragalus radical， and the protein content of young root after treatment was determined. The results showed that the protein content of juvenile root protein of astragalus was rapidly decreased with the extension of toxin stress time. The soluble sugar content of young root of astragalus decreased rapidly after toxin stress， indicating that the respiration of the plant was strengthened. Proline content increased with the increase of stress time and toxin concentration， indicating that astragalus seedling showed sensitive response to toxin damage.

S567.23

甘肃省农业科学院农业科技创新专项“甘肃地区黄芪根腐病优势病原毒素致病机理研究”(2013GAAS41)。