COBRA基因编码糖基磷脂酰肌醇锚定蛋白，是次生壁加厚和纤维素含量的调节因子。亚麻纤维为韧皮纤维，纤维产量与次生壁中纤维素含量及次生壁加厚密切相关。通过BLASTP和Pfam分析，鉴定到24个亚麻COBRA家族成员，并对其进行基因结构、蛋白理化性质及系统进化等生物信息学分析。结果表明，有22个成员具有跨膜结构域，21个成员具有N-端信号肽，16个成员具有GPI锚定位点，所有蛋白均具有CCVS保守结构域而且都定位在细胞膜。基因结构和系统进化分析表明，亚麻COBRA基因家族分为2个亚家族:COBRA-I和COBRA-II，2个亚家族的基因结构具有明显的差异。

The COBRA gene encodes the glycosylphosphatidylinositol anchoring protein， which is a regulator of secondary wall thickening and cellulose content. Flax fiber is bast fiber， and fiber yield is closely related to the content of cellulose in secondary wall and the thickening of secondary wall. Through BLASTP and Pfam analysis， 24 members of the COBRA family were identified in this study， and the gene structure， physicochemical properties of proteins， systematic evolution were conducted. The results showed that 22 members had transmembrane domain， 21 members had N-terminal signal peptide， 16 members had GPI anchor site， and all proteins had CCVS conserved domain and were located in cell membrane. The results of gene structure analysis and systematic evolution showed that the COBRA gene family was divided into two subfamilies， COBRA-I and COBRA-II， the gene structure of the two subfamilies were significantly different. This study laid a foundation for the in-depth study on the function of COBRA gene and the use of COBRA gene to increase the fiber yield.

S565.9

国家自然科学基金项目(31760426)资助。