以温室盆栽的铁皮石斛茎切段为外植体，采用交叉分组设计方法，在MS培养基中，添加不同浓度的植物生长调节剂2.4-D、NAA、IBA和6-BA诱导铁皮石斛丛生芽并观察对丛生芽增殖和生根的影响。结果表明，铁皮石斛丛生芽诱导的最适培养基为MS＋6-BA 2.0 mg/L＋2.4-D 1.0 mg/L；最适丛生芽增殖的培养基为MS＋6-BA 1.5 mg/L＋NAA 1.0 mg/L；最适生根壮苗培养基为1/2 MS＋IBA 1.5 mg/L，移栽基质为珍珠岩、森林腐叶土、松针叶质量比1∶1∶1。

The top bud of Dendrobium officinale potted in greenhouse were used as explants， the method of cross-grouping design was adopted. and cultured in MS + mashed bananas puddle. The different concentrations of plant growth regulators 2.4-D， NAA， IBA and 6-BA were added in MS medium to induce the bushy buds of Dendrobium officinalis， and the effects on the proliferation and rooting of bushy buds were observed. The results showed that the best medium for inducing cluster buds of Dendrobium officinale were MS+6-BA 2.0 mg/L+2.4-D 1.0 mg/L and MS+6-BA 1.5 mg/L+NAA 1.0 mg/L. The optimum medium for multiplication of cluster buds was MS+6-BA 1.5 mg/L+NAA 1.0 mg/L. The optimum medium for multiplication of cluster buds was MS+6-BA 1.5 mg/L+NAA 1.0 mg/L. The transplanting matrix was perlite， forest leaf mould and pine needle mass ratio 1∶1∶1.

S813.1