研究了不同氮素水平对基质盆栽娃娃菜的基质微生物数量、酶活性以及养分含量的影响。结果表明，在施磷、钾肥量相同条件下，随着生育期的推进，栽培基质的氨化细菌数量除施尿素26 g/株处理外，其他处理均降低；硝化细菌、亚硝酸细菌及反硝化细菌数量均增加，且氮素水平对这几种微生物数量化的影响不显著。施氮处理下过氧化氢酶活性显著高于不施氮处理；莲座期追肥后，施尿素8 g/株处理下的基质纤维素酶、脲酶具有较高的活性，而过氧化氢酶和蔗糖酶分别在施尿素36 g/株和施尿素16 g/株处理下达到最大值。不同施氮量对基质中全磷和有效磷含量无显著影响，随施氮量的增加，全钾、速效钾含量呈先增加后降低趋势，在施尿素26 g/株处理下达到最大值。植株地上部全氮、全磷、全钾含量均高于地下部，且均在施尿素26 g/株处理下达到最大值，全氮和全钾显著高于不施氮处理。综上所述，基质盆栽条件下，娃娃菜施肥方案选用尿素26 g/株、硫酸钾38 g/株、普通过磷酸钙34 g/株，有利于提高基质土壤酶活性、基质和植株中养分含量，促进植株养分向地上部运输。

The pot experiment was conducted to study effects of different nitrogen levels on microbial quantity，enzyme activity and nutrient content in mini Chinese cabbage (Brassica pekinensis) cultivated with substrate. The results showed that with the same application of phosphorus and potassium fertilizer， with the advance of growth period， the quantity of ammoniated bacteria in the substrate of a. ambrosia cultivation decreased with the addition of urea treatment at 26 g/plant. The number of nitrifying bacteria， nitrite bacteria and denitrifying bacteria increased， and the influence of nitrogen level on the quantification of these microorganisms was not significant. Catalase activity was significantly higher under nitrogen application than that under no nitrogen application. After topdressing at lotus stage， matrix cellulase and urease had higher activity under urea application of 8 g/plant， while catalase and sucrase reached their maximum values under urea application of 36 g/plant and 16 g/plant， respectively. With the increase of nitrogen application rate， the total and available potassium contents increased first and then decreased， and reached the maximum under the treatment of urea 26 g/plant. The contents of total nitrogen， total phosphorus and total potassium in the shoot were higher than those in the underground， and reached the maximum under the treatment of 26 g/plant urea per plant. The contents of total nitrogen and total potassium were significantly higher than those under the treatment of no nitrogen application. In summary， under the condition of pot culture with substrate， the fertilization schemes of Mini Chinese cabbage include 26 g/plant urea per plant， 38 g/plant potassium sulfate per plant and 34 g/plant common superphosphate per plant， which are beneficial to improving soil enzyme activity， nutrient content in matrix and plant， and promoting nutrient transport from plant to shoot.

S634.3；Q81

甘肃农业大学大学生科研训练项目(20181209)；甘肃省自然科学基金(18JR3RA166)；甘肃农业大学人才专项(GSAU-RCZX201713)；甘肃农业大学盛彤笙科技创新基金(GSAU-STS-1746)；甘肃省科技重大专项(17ZD2NA015-03)。