[关键词]
[摘要]
为了探究NaCl胁迫对胡麻幼苗生长及其离子吸收运输的影响,采用水培法对胡麻幼苗的盐分离子吸收、分布、累积特性进行了研究。结果表明,胡麻幼苗干鲜重、含水量、叶绿素含量均在NaCl低浓度(≤100 mmol/L)胁迫下小幅上升,并随着胁迫浓度的增加而下降。地上和地下组织中膜透性呈现出明显的上升趋势,游离脯氨酸含量和可溶性蛋白含量均随着NaCl浓度的增加表现为先上升后缓慢下降。随着NaCl浓度的增加根茎叶中Na+、Cl-含量均为上升趋势,其中Na+积累顺序为叶 > 根 > 茎,Cl-积累顺序为根 > 茎 > 叶,Ca2+流失顺序为根 > 茎 > 叶,K+流失顺序为叶 > 根 > 茎。高浓度盐胁迫下胡麻幼苗将相对较多的Na+ 积累在叶片中,导致叶片中K+流失较根茎中更严重,Ca2+流失首先从根部开始,以延缓茎和叶中的流失,起到缓解盐害目的。
[Key word]
[Abstract]
To study the influence of NaCl stress on the growth and ions transport of flax seedlings, the absorption, distribution and accumulation of saline ions in seedlings of flax were analysed by water culture experiment. The results showed that the fresh and dry weight, the water and chlorophyll content of flax seedlings all increased slightly under NaCl(≤100 mmol/L) stress, and decreased with increasing concentration of NaCl. Whether above-ground or under-ground tissue of flax seedlings, the membrane permeability increased, but the contents of free proline and soluble protein all increased firstly and then decreased slowly with increasing NaCl concentration. The content of Na+ and Cl- in the roots, stems and leaves of flax seedlings all showed increasing trend with increasing NaCl concentration, the accumulation of Na+ and Cl- were in ascending order: stem, root, leaf, and leaf, stem, root, respectively. The loss of Ca2+ and K+ were in ascending order: leaf, stem, root, and stem, root, leaf, respectively. More Na+ were accumulated in leaves of flax seedlings under higher salt stress, which resulted in the more loss of K+ from leaves; the loss of Ca2+ started from roots, which could delay the loss from stem and leaves and then alleviate the salt damage.
[中图分类号]
S512.1
[基金项目]
国家自然科学基金项目(31560347、31660368、31760426);兰州市人才创新创业项目(2017-RC-97);甘肃省现代农业产业技术体系建设专项(GARS-07-05);甘肃省农业科学院科研条件建设及成果转化项目(2017GAAS22)资助。