以武山县塑料大棚黄瓜连作10 a的土壤为对象，研究了放线菌制剂对大棚黄瓜土壤微生物群落结构和酶活性的影响。结果表明，除结果期外，与不施放线菌处理相比，穴施2 g放线菌制剂时，硝化细菌、亚硝化细菌、纤维素厌氧分解菌数量显著升高，土壤真菌数量则呈下降趋势，在拉秧期降低了48.86%；穴施4 g放线菌制剂的土壤蔗糖酶活性显著升高。说明放线菌制剂对大棚黄瓜连作土壤的微生态环境有明显的改善效果。

The test took the soil which had grew cucumber for ten years as materials in Wushan County greenhouse， the effects of actinomycetes on microbial community structure and enzyme activity in greenhouse cucumber soil were studied. The results showed that compared with the non-actinomycetes treatment， in addition to the fruit stage， the number of nitrifying bacteria， nitrite bacteria and cellulose anaerobic decomposing bacteria increased significantly when 2 g actinomycetes were applied to the hole， while the number of soil fungi showed a decreasing trend， which decreased by 48.86% at the seedling rearing stage；the soil sucrase activity of 4 g actinomycetes was significantly increased. The results showed that actinomycetes could improve the microecological environment of cucumber continuous cropping soil.

S642.2；S154.3

甘肃省农业科学院科技支撑计划(2017GAAS41)；甘肃省农业科学院“土壤培肥及退化修复”科技创新团队(2015GAAS03)；公益性行业(农业)科研专项(201503120)；甘肃省农业科学院科技创新专项计划(2017GAAS28)资助。