采用生长速率法测定了5种生物源农药对梨树腐烂病菌菌丝生长的抑制效果。结果表明，除10%烟碱水剂外，其他4种生物源农药对梨树腐烂病菌均具有显著的抑制作用。当0.3%丁子香酚可溶液剂、0.3%苦参碱乳油、0.4%蛇床子素可溶液剂、0.5%小檗碱水剂质量浓度分别为0.500 00、2.500 00、2.500 00、5.000 00 μg/mL时，其对梨树腐烂病菌菌丝生长的抑制率分别为94.9%、98.9%、94.5%和96.9%，EC50分别为0.083 0、0.144 9、0.233 9、0.268 7 μg/mL；10%烟碱水剂的室内毒力最差，EC50为6 344.617 0 μg/mL。综上所述，0.3%丁子香酚可溶液剂、0.3%苦参碱乳油、0.4%蛇床子素可溶液剂、0.5%小檗碱水剂对梨树腐烂病室内毒力较好。

In this study， the inhibitory effects of five different biological source pesticide on mycelial growth by the method of mycelial growth rate. The results showed that except for 10% nicotine AS were the lowest toxic， four kinds of biological source pesticide had siginificant inhibitory effects on the growth of Valsa mali var. pyri. when the mass concentration of eugenol 0.3% SL， matrine 0.3% EC， osthole 0.4% SL， berberine 0.5% AS were 0.500 00， 2.500 00， 2.500 00 and 5.000 00 μg/mL， the inhibitation rate on mycelial growth were 94.9%、98.9%， 94.5% and 96.9%， and the EC50 values were 0.083 0、 0.144 9、0.233 9、0.268 7 μg/mL respectively. Nicotine 10% AS were the lowest toxic， EC50 values were 6 344.617 0 μg/mL. In summary， among the five kinds of biological source pesticide， eugenol 0.3% SL， matrine 0.3% EC， osthole 0.4% SL， berberine 0.5% AS had good inhibitory effects.

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国家重点研发计划(2018YFD0201404-4)；公益性行业(农业)科研专项(201203035)；甘肃省现代水果产业技术体系(GARS-SG-2)；甘肃省科技支撑计划(1204NKCA099)。