采用工厂化双孢蘑菇隧道发酵工艺进行培养料制备，将制备后的培养料制作不同投料量的菌包，在相同栽培管理条件下对比双孢蘑菇的菌丝长势、出菇期、产量和效益。结果表明，采用投料量为22 kg/包的菌包，单菇重、商品菇率均优于20 kg/包的菌包。产量为18.97 kg/m2，比投料量20 kg/包的菌包增产1.45 kg/m2，增产率8.28%；纯收入达40.68元/m2，比投料量20 kg/包的菌包增加2.57元/m2，增值6.74%。22 kg/包可作为双孢蘑菇农法栽培最佳的菌包发酵料投料量。

The culture material was prepared by the industrialized tunnel fermentation technology of Agaricus bisporus， and then used to make the fungi bags with different amount of materials. The comparative test of mycelium growth， fruiting period， yield and benefit of Agaricus bisporus under the same cultivation and management conditions. The results showed that the inventory was 22 kg/bale of fungi bag， the weight of single mushroom and the rate of commercial mushroom were better than those of 20 kg/bale. The yield was 18.97 kg/m2， which increased by 1.45 kg/m2 compared with the 20 kg/bale inoculation， and the yield increase rate was 8.28%. The net income reaches 40.68 yuan/m2， which increased by 2.57 yuan/m2 compared with the 20 kg/bale bacteria bag， with an increment of 6.74%. It can be used as the best fermentation material for Agaricus bisporus farming culture.

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国家现代农业产业技术体系专项(CARS-24)；甘肃省农业科学院创新专项食用菌遗传育种与高效栽培学科团队(2017GAAS32)。