[关键词]
[摘要]
以绵羊RXRG基因为目的基因,利用生物信息学软件预测其结构和功能。结果表明,绵羊RXRG基因编码463个氨基酸,开放阅读框长度为1 392 bp,起始密码子位于228 bp处,终止密码子位于1 619 bp处。RXRG基因编码蛋白的相对分子质量为50 845.19 Da,等电点为7.55,在氨基酸组成中亮氨酸所占比率最高,色氨酸占比最低。亚细胞定位主要位于细胞核中,不属于分泌蛋白;不存在信号肽序列;存在两个保守结构域,并且为疏水性蛋白,二级结构主要以α螺旋和无规则卷曲为主,三级结构主要由无规卷曲缠绕折叠形成。
[Key word]
[Abstract]
In this study, with the sheep RXRG gene as the target gene, the structure and function of sheep RXRG gene were predicted by bioinformatics software. The RXRG gene of sheep encodes 463 amino acids, the length of open reading frame is 1 392 bp, the initial codon is 228 bp, and the termination codon is 1 619 bp. The relative molecular weight and isoelectric point of RXRG protein were 50 845.19 Da and 7.55 respectively. Leucine accounted for the highest proportion of amino acid composition and tryptophan accounted for the lowest proportion. Subcellular localization is mainly located in the nucleus, not in the secretory protein;there is no signal peptide sequence;there are two conservative domains, and they are hydrophobic proteins. The secondary structure is mainly αshelix and irregular curl, and the third structure is mainly formed by random curl winding and folding.
[中图分类号]
S826
[基金项目]
甘肃农业大学科技创新基金(GAU-XKJS-2018-023)。