以8叶龄垂丝海棠实生苗为试验材料，通过喷施不同浓度的葡萄糖与蔗糖，研究外源糖对其缺铁胁迫下生理的影响。结果表明，垂丝海棠叶片的相对叶绿素(SPAD)含量、相对含水量、超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、亚铁离子浓度(Fe2+)在 -Fe处理下相比于对照显著下降，在外施葡萄糖和蔗糖后，其含量相比于-Fe处理显著上升，且具有浓度效应，随处理浓度的增加呈先升后降趋势；而脯氨酸(Pro)含量随着处理浓度的增加呈上升趋势，各处理的Pro含量均显著高于对照。此外，缺铁处理下丙二醛(MDA)相比于对照显著上升，在外施葡萄糖和蔗糖后其升幅显著下降，且存在浓度效应，随处理浓度的增加呈先升后降趋势。依据主成分得分排序，外源葡萄糖和蔗糖对垂丝海棠缺铁胁迫缓解能力由高到低均为:CK ＞ -Fe+2 ＞ -Fe+4 ＞ -Fe+1 ＞ -Fe+8 ＞ -Fe。研究发现，2 mM的外源葡萄糖和蔗糖均能通过调节渗透物质、增强抗氧化酶活性、增加生物膜的稳定性、提高亚铁离子含量以达到缓解缺铁胁迫的效应，且蔗糖处理的缓解效应更好。

Malusa halliana seedlings of 8 leaf age were used as experimental materials to study the effects of exogenous sugar on physiological characteristics of apple rootstock under iron deficiency stress by sprayed with different concentrations of glucose and sucrose. The results showed that the vertical wire relative haitang leaf chlorophyll content(SPAD)， relative water content， superoxide dismutase(SOD)， peroxidase(POD)， catalase(CAT)， concentration of ferrous ions(Fe2+) under -Fe treatment compared with the control(CK) decreased significantly， after the glucose and sucrose， compared with its content -Fe a marked increase in the processing， and has the concentration effect， with the increase of the concentration showed a trend of rose first and then fell. Proline content(Pro) showed an increasing trend with the increase of treatment concentration， and the Pro content of each treatment was significantly higher than CK. In addition， malondialdehyde(MDA) significantly increased when compared with CK under iron deficiency treatment， and its increase significantly decreased after the application of glucose and sucrose， and there was a concentration effect， which a trend of rose first and then fell with the increase of the treatment concentration. According to the order of principal component score， the ability of exogenous glucose and sucrose to alleviate the iron deficiency stress of malus tuber from high to low was: CK > -Fe+2 > -Fe+4 > -Fe+1 > -Fe+8 > -Fe. It was found that both 2 mM exogenous glucose and sucrose could alleviate the effect of iron deficiency stress by regulating osmotic substances， enhancing the activity of antioxidant enzymes， increasing the stability of biofilm， and improving the content of ferrous ions， and sucrose treatment had better mitigative effect.

S661.4

甘肃农业大学学生科研训练计划项目(S202010733083)。