通过正交试验优化了党参中醇溶性多糖的提取工艺，并对其生物活性进行了研究。最佳提取条件固液比为1∶20，提取温度为65 ℃，提取时间为4.5 h，为超声波辅助提取，提取次数为2次，在这些条件下，党参多糖的产率为26.25±1.02％。分析表明，党参多糖中，总糖、蛋白质、糖醛酸含量分别为805、31～66、2.1 g/kg。体外抗氧化实验结果表明，在浓度为2 mg/mL时，党参多糖对ABTS+、DPPH、和HO-具有良好的清除作用。对不同区域党参多糖进行含量比较，认为降水量充足、冷凉的气候、较高的海拔有利于糖分积累。

The extraction process of alcohol-soluble polysaccharide on Codonopsis pilosula was optimized by orthogonal tests， and its biological activity was studied. The optimal extraction conditions were the ratio of solid-to-liquid was 1∶20 g/mL， temperature 65 ℃， extraction time 4.5 h， and ultrasonic assisted extraction cofactor and extraction number was two times， polysaccharide yield reached 26.25±1.02%. Chemical composition analysis showed that the total sugar， protein and glyuronate content of Codonopsis pilosula polysaccharide were 805 g/kg， 31～66 g/kg and 2.1 g/kg， respectively. In vitro antioxidant assays demonstrated that Codonopsis pilosula polysaccharide had good clearance on ABTS+， DPPH and HO- at a concentration of 2 mg/mL. By comparing the content of codonopsis pilotica polysaccharide in different regions， sufficient rainfall， cold climate and high altitude were conducive to polysaccharide accumulation.

S567.5

国家重点研发项目(2018YFD0201100)；甘肃省重点人才项目(2019RCXM072)；甘肃省农业科学院重点研发计划(2020GAAS30、2020 GAAS25)。