[关键词]
[摘要]
LEC基因家族包含LEC1和LEC2,参与植物的生长发育及储藏物质的积累。LEC1(Leafy Cotyledon 1)是NF-YB9蛋白家族的成员,编码了CCAAT-box结合转录因子HAP3(Heme activated protein 3)亚单位。利用生物信息学方法,获得了16个玉米LEC1基因。分析发现,ZmLEC1蛋白大部分都是酸性的不稳定亲水蛋白,含有1个保守结构域CBFD_NFYB_HMF及10个保守基序。通过与拟南芥LEC1基因和水稻LEC1基因的聚类分析,将玉米LEC1基因家族分为4类:ClassⅠ、ClassⅡ、ClassⅢ、ClassⅣ。经RNA-seq等分析发现,玉米LEC1家族基因具有组织特异性,响应非生物胁迫。启动子分析发现,玉米LEC1基因启动子上含有响应激素和非生物胁迫的顺式作用元件。
[Key word]
[Abstract]
The LECfamily genes, consisting of LEC1 and LEC2, are involved in the development and accumulation of storage materials in plants. LEC1(Leafy Cotyledon 1) is a member of the NF-YB9 protein family, encoding the CCAAT-box binding transcription factor HAP3 (Heme activated protein 3) subunit. In this study, we identified 16 LEC1 genes by using bioinformatics. Most of the ZmLEC1 proteins are acidic unstable hydrophilic proteins, containing a conserved domain CBFD_NFYB_HMF and 10 conserved motifs. By aligning the sequence of LEC1 genein Arabidopsis and rice, respectively, these genes were divided into four categories as ClassⅠ、ClassⅡ、ClassⅢ、ClassⅣ. According to the analysis of RNA-seq, ZmLEC1 preferentially expressed in specific tissues, responding to the abiotic stress. Promoter region analysis revealed that the promoter of ZmLEC1 contained cis-acting elements in response to hormonal and abiotic stresses.
[中图分类号]
S513
[基金项目]
国家自然科学基金面上项目(31771801、32072069);湖北省高等学校优秀中青年创新团队项目(2017T04)。