高迁移率族蛋白A1基因(high mobility group protein A1，HMGA1)是一类对DNA转录起调控作用的基因，广泛存在于多种动物体内。为探究HMGA1基因在绵羊体内的功能，对该基因及其编码产物进行了生物信息学分析。结果显示，绵羊HMGA1基因编码152个氨基酸残基，其编码的蛋白质分子式为C443H759N151O151S1，分子质量为10.648 35 kDa，等电点pI为10.31。绵羊HMGA1蛋白为不稳定蛋白、亲水性蛋白、非分泌蛋白且无信号肽序列和跨膜结构；亚细胞定位主要存在于细胞核(95.7%)。绵羊与牛的HMGA1蛋白相似度为100%。其二级结构和三级结构主要以无规卷曲组成。

High mobility group protein A1(HMGA1) gene is a kind of gene that regulates DNA transcription and exists widely in many kinds of animals. In order to explore the function of HMGA1 gene in sheep， bioinformatics analysis of HMGA1 gene and its encoding product was conducted in this study. The results showed that sheep HMGA1 gene encoded 152 amino acid residues， and its protein molecular formula was C443H759N151O151S1， molecular weight was 10.64 835 Kda， isoelectric point pI was 10.31. Sheep HMGA1 protein was an unstable protein， hydrophilic protein， non-secretory protein and has no signal peptide sequence and transmembrane structure. The localization of HMGA1 at a subcellular level was mainly existed in the nucleus (95.7%). The HMGA1 protein similarity between sheep and cattle was 100%. Its secondary and tertiary structures were mainly composed of random crimp.

S826；Q57

甘肃农业大学学生科研训练计划项目(202104026)。