利用兰州百合与大花卷丹进行正反交，研究了花期调控、不同授粉方式、蒴果采收时间及胚挽救培养基配方对杂种胚萌发率及成苗率的影响。结果表明，兰州百合延后种植及大花卷丹提前种植，可有效地解决花期不遇的问题；兰州百合与大花卷丹杂交授粉时，花期授粉的蒴果膨大率可达93.3%，种子有胚率为2.00%；大花卷丹与兰州百合杂交授粉时，切割柱头的授粉膨大率最高，仅为7.6%，但种子有胚率为0%。兰州百合与大花卷丹杂交授粉后60～70 d的蒴果进行胚培养较为适宜。兰州百合与大花卷丹的杂交胚适宜培养基为MS+0.3 mg/LNAA+0.2 mg/L6-BA。

The effects of flowering regulation， different pollination methods， capsule harvesting time and embryo rescue medium formula on the germination rate and seedling rate of hybrid embryos were studied by positive and negative cross between Lilium davidii var. unicolor and Lilium leichtlinii var. maximowiczii. The results showed that delayed planting at different time points of low temperature storage during cold storage of Lilium davidii var. unicolor and early planting protection measures of Lilium leichtlinii var. maximowiczii could effectively resolve the problem of flowering； Flowering pollination can be used in hybrid pollination of Lilium davidii var. unicolor × Lilium leichtlinii var. maximowiczii， capsule expansion rate can reach 93.3%， the embryo rate of seeds was 20‰； while Lilium leichtlinii var. maximowiczii hybrided with Lilium davidii var. unicolor， the expansion rate of cutting stigma pollination was the highest， only 7.6%， but the embryo rate of seeds was 0%； It was more suitable for embryo culture of capsules 60 d~70 d after pollination by Lilium davidii var. unicolor × Lilium leichtlinii var. maximowiczii； the optimum medium for the hybrid embryos of Lilium davidii var. unicolor hybirded with Lilium leichtlinii var. maximowiczii was MS+0.3 mg/L NAA+0.2 mg/L 6-BA.

S644.1

兰州市人才创新创业项目(2020-RC-144)；陇原青年创新创业人才项目(2022)。