为进一步探索绵羊APOA4基因及其编码蛋白的生物学功能，给APOA4基因与绵羊健康和生长发育的关系提供依据，以绵羊载脂蛋白A-IV(Apolipoprotein A-IV，APOA4)基因为研究对象，采用生物信息学数据库及分析软件进行生物信息学分析，预测绵羊APOA4基因基本结构、理化特性和生物学特征。结果显示，绵羊APOA4基因ORF编码380个氨基酸残基，编码蛋白为不稳定亲水蛋白，存在信号肽，不存在跨膜结构，为分泌性蛋白，主要在细胞外发挥生物学作用，二级结构和三级结构均以α-螺旋为主。绵羊APOA4基因编码蛋白与山羊和牛的同源性最高。

To further explore the Apolipoprotein A-IVgene(APOA4) functions and the biological characteristics of its encoded protein in sheep and to provide reference for the relationship between APOA4 and sheep health and development, in this study, the basic structure, physical and chemical characteristics and biological characteristics of sheep APOA4 were predicted by bioinformatic database and analysis software. The results showed that the ORF of sheep APOA4 encoded 380 amino acids. The protein encoded by APOA4 was an unstable hydrophilic protein with signal peptide but no transmembrane structure. It's a secretory protein, which mainly played a biological role in extracellular cells. Both secondary structure and tertiary structure were dominated by α-helix. Sheep APOA4 had the highest homology with that in the goat and the cattle.

S826

国家自然科学基金地区科学基金项目(31660670)。