核糖体蛋白20基因(ribosomal proteinS20，RPS20)是一类参与蛋白质生物合成及对细胞增殖、分裂、分化、凋亡具有调控作用的基因，广泛存在于多种动物体内。为探究RPS20基因在绵羊体内的功能，运用生物信息学数据库及其软件分析了该基因及其编码的产物。结果发现，绵羊RPS20基因总共编码了119个氨基酸残基，所编码的蛋白质分子式为C587H995N173O171S5，分子质量为13.372 71 KDa，等电点pI为9.95。通过基本理化性质的分析可知，绵羊RPS20蛋白是稳定性强、具亲水性的非分泌蛋白，不含信号肽序列且无跨膜结构。亚细胞定位结果表明，其编码产物主要存在于细胞质中(65.2%)。绵羊与牛、马、黑猩猩、野生双峰驼等哺乳动物的RPS20蛋白相似度均为100%。该蛋白的二级结构和三级结构主要以无规卷曲、α螺旋和β折叠组成。

Ribosomal protein S20(RPS20) gene is a class of genes involved in protein biosynthesis and regulating cell proliferation, division, differentiation and apoptosis, which is widely present in a variety of animals. In order to explore the function of RPS20 gene in sheep, bioinformatic analysis of RPS20 gene and its encoded products was conducted by using bioinformatic databases and software. The results showed that RPS20 gene encoded 119 amino acid residues, the protein molecular formula was C587H995N173O171S5, the molecular weight was 13.37 271 KDa, isoelectric point pI was 9.95. The RPS20 protein was a stable, hydrophilic, non-secretory protein and that contained no signal peptide sequence and no transmembrane structure. Subcellular localization mainly existed in the cytoplasm (65.2%). The similarity of RPS20 proteins among sheep and mammals such as cattle,horses, chimpanzees, and wild bactrian camels was 100%. Its secondary and tertiary structure were mainly composed of random curl, α helix and β fold.

S826

甘肃农业大学学生科研训练计划项目(202204059)。