为了明确扁蓿豆是否存在自毒效应，以期为扁蓿豆科学种植提供理论依据。以扁蓿豆种子为受体，采用室内培养皿种子萌发方法，研究了不同质量浓度(0、0.001、0.025、0.050、0.100 g/mL)扁蓿豆(Medicago ruthenica)地上部分和地下部分水浸提液的自毒作用。结果表明，扁蓿豆植株地上部分与根部水浸提液对其种子发芽率、发芽势、发芽指数、胚根长、胚芽长和单株鲜质量具有一定的抑制作用，总体表现为在质量浓度0.100g/mL下抑制作用最强。扁蓿豆地上部分水浸提液处理在0.100 g/mL质量浓度下发芽率较对照蒸馏水显著降低了39.00%(P < 0.05)，种子发芽指数较对照蒸馏水降低了82.00%；根部水浸提液在0.100 g/mL质量浓度下胚根长较对照蒸馏水显著降低了50.00%。各指标的化感效应指数与质量浓度均呈负相关。

To illustrate the autotoxicity in Medicago ruthenica seeds and to provide theoretical reference for the cultivation of Medicago ruthenica, the autotoxicity of different concentrations(0, 0.001, 0.025, 0.050 and 0.100 g/mL) of aqueous extracts from aboveground part and root of Medicago ruthenica were studied by indoor petridish seed germination method in laboratory. Results showed that the aqueous extracts from the aboveground part and root of M. ruthenica showed certain inhibitory effects on the seed germination rate, germination potential, germination index, radicle length, germ length and fresh quality of a single plant. The overall inhibitory effect was the strongest at the concentration of 0.100 g/mL. At the concentration of 0.100 g/mL, the germination rate using water extract aboveground was 39.00% lower than that of CK, i.e. distilled water only(P < 0.05), and the seed germination index was 82.00%lower than that of CK, at the concentration of 0.100 g/mL, the length of hypocotyl root using water extract from root was significantly reduced by 50.00% compared with that of CK, and the allelopathic effect index of each parameter was negatively correlated with the concentration.

S634.5

甘肃农业大学大学生创新创业训练计划项目(202102043)。