为获得大花卷丹百合的优质种苗，促进其规模化生产，以大花卷丹百合的鳞片为外植体，采用MS、N6、B5培养基，添加激素NAA、6-BA、GA3，进行了大花卷丹百合的快繁技术研究。结果表明，外植体在N6培养基中产生愈伤组织最快、效果最好。愈伤组织及不定芽诱导最佳培养基配方为N6+1.0 mg/L 6-BA+0.1 mg/L NAA；不定芽增殖最佳培养基为N6+0.5 mg/L 6-BA+0.1 mg/L NAA+1.0 mg/L GA3。NAA诱导小鳞茎效果最佳，最适培养基为 N6+1.0 mg/L NAA；促进小鳞茎膨大生根要在诱导鳞茎的基础上加入IBA，即最适培养基为N6+0.1 mg/L NAA+2.0 mg/L IBA。

To obtain the premium seedlings of Lilium leichtlinii var. maximowiczii(Regel) Baker and to promote its large-scale production, using scales as the explant, MS, N6 and B5 media with the addition of NAA, 6-BA and GA3 were applied to conduct the study on techniques of rapid propagation for the tissue culture of Lilium leichtlinii var. maximowiczii(Regel) Baker. Results showed that N6 medium was the quickest and the best medium to induce the callus formation, optimum medium for the induction of callus and adventitious buds was N6 plus 1.0 mg/L 6-BA plus 0.1 mg/L NAA. The optimum medium for adventitious bud proliferation was N6 plus 0.5 mg/L 6-BA plus 0.1 mg/L NAA plus 1.0 mg/L GA3. NAA showed the best effect in inducing small bulblets and the optimum medium was N6 plus 1.0 mg/L NAA whereas IBA ought to be added to promote the rooting of bulblet enlargement with the optimum medium selected as N6 plus 1.0 mg/L NAA plus 2.0 mg/L IBA.

S682.2

吉林省科技厅科技计划项目(212566YY010286378)。